Mark D. Lazzaro - Published Videos
These videos are supplements to figures in this publication and are also published on the journal website.
Lazzaro MD, Cardenas L, *Bhatt AP, *Justus CD, *Phillips
MS, Holdaway-Clarke TL, Hepler PK (2005) Calcium Calcium
gradients in conifer pollen tubes; dynamic properties
differ from those seen in angiosperms. Journal of
Experimental Botany 56:2619-2628.
Figure 1: Pollen tubes
have a 2-fold calcium gradient that pulsates as organelles
move in a fountain pattern into the tip. (2 MB)
Figure 3: BAPTA injection
triggers a dramatic pulsating influx of calcium.
Vesicles accumulate in the tip as growth stops. (8 MB)
Figure 4: Caffeine
dissipates the calcium gradient triggering a large
transient influx of calcium that disrupts motility. (9 MB)
Figure 6: Lanthanum
dissipates the calcium gradient but does not affect
motility. During recovery elevated calcium influx occurs
which causes the fountain pattern of organelle motility to
switch direction to a reverse fountain. (15 MB)
Figure 7: Verapamil stops
organelle motility and growth as vesicles accumulate at
the tip. During recovery the fountain pattern returns but
growth does not resume. (11 MB)
